Fluidigm Showcases RNA Sequencing Innovations Using Microfluidics Technology at the 2019 American Society of Human Genetics (ASHG) Meeting
"As the demand for RNA NGS sequencing continues to rise, many genomics laboratories have found it challenging to affordably scale NGS library preparation," said Chris Linthwaite, President and CEO of Fluidigm. "This year at ASHG, we are excited to showcase a breakthrough automated RNA sequencing library preparation workflow that enables laboratories to efficiently produce 48 libraries at a time while substantially reducing their cost per sample. By automating the library prep workflow, shortening hands-on time and decreasing consumables waste, we can bring operational savings to mid-to-high-throughput laboratories using microfluidics technology that could amount to hundreds of thousands of dollars.”
Featured RNA Sequencing and Genomic Applications
- The Advanta™ RNA-Seq NGS Library Prep Kit enables researchers to reduce their costs for full-length stranded libraries by up to 50% using microfluidics technology. Developed for use with the Fluidigm Juno™ microfluidic system, the kit enables an automated workflow that requires only 10 ng of total RNA and can reduce operator interventions and total hands-on time to approximately two hours, a decrease of more than 70% as compared to traditional manual methods.
Results using the
Advanta RNA-Seq Library Prepworkflow with samples down to 1 ng total RNA will be presented on Friday, October 18, by Michael L. Phelan, PhD, of Fluidigm in a poster titled, “Integration of molecular chemistries supporting a full-length mRNA sequencing library preparation method on a microfluidic circuit” (poster 1874F).
- The Advanta RNA Fusions NGS Library Prep Assay profiles over 1,000 known gene fusion breakpoints present in tumor RNA, automating library preparation for subsequent analysis on
Illumina® NGS platforms. Requiring as little as 10 ng of RNA material per sample, the automated microfluidic workflow reduces hands-on time and lowers reaction volumes to nanoliter scale, delivering significant cost savings per samples. Designed to work in coordination with the Advanta Solid Tumor NGS Library Prep Assay in a single, simple workflow, the RNA Fusions Assay enables researchers to prepare NGS libraries for up to six different panels at a time with 10-fold lower sample input.
Performance using both Advanta oncology assays will be presented on Friday, October 18, by Tom Goralski, PhD, of Fluidigm in a poster titled, “Two NGS library prep panels for analyzing tumor cells for actionable mutations using a flexible, automated microfluidics platform” (poster 1754F).
- The C1™ system provides a 360-degree view of single cells with the widest menu of unique multi-omic applications including co-detection of mRNA with noncoding RNAs, protein expression, DNA and more. Using proprietary microfluidics technology, C1 captures and processes each single cell within complex enriched or non-enriched cell populations, maximizing molecular detection sensitivity. When using C1 together with the C1 Open App™ application, researchers also have the unique ability to build new innovative applications that go beyond conventional single-cell mRNA sequencing.
Exhibitor Workshops Utilizing Fluidigm Microfluidics Technology
- On Wednesday, October 16, Fluidigm will host a lunch workshop titled
Redefining Library Prepfor RNA-Seq Applications–A Microfluidics-Based Approach for Superior Workflow Efficiencies. David King, PhD, Vice President, Genomics Researchand Development, will provide an overview of the development of the Advanta RNA-Seq NGS Library Prepsystem. Scott Magness, PhD, Associate Professor at the University of North Carolina School of Medicine, will present results using the Advanta Library Prepworkflow to detect change in gene expression in response to hypoxia.
Thursday, October 17, Fluidigmwill host a breakfast workshop titled Moving Beyond Classification of Single Cells in Complex Systems Leveraging Microfluidics. Jason McKinney, Director of Single-Cell Cell Genomics, will describe a total RNA-seq approach for automated NGS library prep of single cells using microfluidics technology, enabling full-length transcript analysis of both poly(A) and non-poly(A) genes while also retaining strand orientation. Additional C1 applications including CORTAD-seq (full-length mRNA and targeted genomic DNA from the same cell) and REAP-seq (mRNA and protein expression from the same cell) will also be presented.
More information on the Fluidigm products and presentations can be found at Fluidigm Booth 1107 or at fluidigm.com/ashg2019.
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Source: Fluidigm Corporation